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Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
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Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
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Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
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Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
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Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
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Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
Origin 2022 Software, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
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Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
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Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
Statistical Package Software Version V.9.9.0.225 (Sr1) Originpro 2022, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss scanning electron microscopy (sem)
Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
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Respira Therapeutics wearable bioimpedance chest patch
Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by <t>HPLC.</t> Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.
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Image Search Results


Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by HPLC. Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.

Journal: Journal of fungi (Basel, Switzerland)

Article Title: Suppression of Chitin-Triggered Immunity by a New Fungal Chitin-Binding Effector Resulting from Alternative Splicing of a Chitin Deacetylase Gene.

doi: 10.3390/jof8101022

Figure Lengend Snippet: Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by HPLC. Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.

Article Snippet: Fungi 2022, 8, 1022 6 of 20 directly analysed using a Jasco high performance liquid chromatography (HPLC) system equipped with a Luna 5 μm NH2 100 Å column (250 × 4.6 mm) (Phenomenex, Madrid, Spain).

Techniques: Binding Assay, Activity Assay, Incubation